Streptococcal M protein: molecular design and biological behavior

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Streptococcal M protein: molecular design and biological behavior.

M protein is a major virulence determinant for the group A streptococcus by virtue of its ability to allow the organism to resist phagocytosis. Common in eucaryotes, the fibrillar coiled-coil design for the M molecule may prove to be a common motif for surface proteins in gram-positive organisms. This type of structure offers the organism several distinct advantages, ranging from antigenic vari...

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Superantigenicity of streptococcal M protein

M proteins that define the serotypes of group A streptococci are powerful blastogens for human T lymphocytes. The mechanism by which they activate T cells was investigated and compared with the conventional T cell mitogen phytohemagglutinin, and the known superantigen staphylococcal enterotoxin B. Although major histocompatibility complex (MHC) class II molecules are required for presentation, ...

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Streptococcal M Protein Extracted by Nonionic Detergent

Streptococcal M proteins are antiphagocytic molecules varying immunologically from type to type while maintaining an identical biological effect. The virulence of the group A streptococcus is directly related to the presence of the M antigens on the cell surface and resistance to infection by these organisms is dependent on the presence of opsonic antibodies directed towards the M molecules. Va...

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Fibrinogen Precipitation by Streptococcal M Protein

Evidence confirming the identity of fibrinogen-precipitating factor and streptococcal M protein is provided by the demonstration of bactericidal, mouse protective, and long chain-producing antibodies in the sera of rabbits immunized with washed M-fibrinogen precipitates. Two precipitin lines were observed in immunoelectrophoresis of human plasma vs. rabbit anti-M-fibrinogen antiserum; no precip...

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Streptococcal M6 protein expressed in Escherichia coli. Localization, purification, and comparison with streptococcal-derived M protein

Type 6 streptococcal M protein produced by E. coli bearing plasmid pJRS42.13 (ColiM6) accumulates in the periplasmic space of this new host. No immunoreactive M protein was found either on the surface of the organism or in the culture medium. The ColiM6 protein was purified from the periplasm and the final preparation consisted of three protein bands of apparent molecular weight 55,000, 57,000,...

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ژورنال

عنوان ژورنال: Clinical Microbiology Reviews

سال: 1989

ISSN: 0893-8512,1098-6618

DOI: 10.1128/cmr.2.3.285